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BIOL 434/BMS 534 Basic Principles and Methods in Molecular Genetics

(Laboratory–Lecture course) Prerequisite: BIOL 316 or permission of the instructor. (First semester/ 3 credits) 

This integrated lab-lecture course provides basic concepts and hands-on experience with common molecular genetics and recombinant DNA methods. Topics include techniques for the isolation of DNA and RNA, gene cloning employing plasmid vectors, DNA sequencing, polymerase chain reaction (PCR) technology, rapid amplification of cDNA ends (RACE), expression of fusion proteins in E. coli, site-directed mutagenesis and web-based analysis of DNA and protein sequence data.